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KMID : 0378019890320010052
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New Medical Journal
1989 Volume.32 No. 1 p.52 ~ p.58
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A Study on the Bio-electrode for Urea utilizing Perteus mirabilis immobilized on a Carbon Doxide Gas-sensor(Ⅰ)
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Abstract
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A potentiometric sensor for urea has been made by immobilizing the bacterium Proteus mirabilis on a carbon dioxide gas-sensor.
This bio-electrode gives good results and selectivity for urea determination.
The response of the bio-electrode is linear range between 7.O x 10-4 and 5.O x 10-2M urea with a response slope of 48 mV/decade at pH 7.0 and 30°C in the 0.1 M phosphate buffer solution. This bio-electro was
investigated for the effects of pH, temperature, buffer solutions, bacteria amounts, interferences, inorganic salts, and the lifetime.
Actually this bio-electrode will be useful for the determination of urea.
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KEYWORD
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